RPMI 1640 Medium, no phenol red.

Does RPMI media have phenol red?

RPMI 1640 Medium, no phenol red.

Is RPMI a basal medium?

RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

What is in RPMI media?

One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)

Is phenol red light sensitive?

Note that phenol red is a chromophore that will absorb light and thus decrease the effective illumination of a sample. More importantly, phenol red can contribute to background and can even mark lysosomes in cells illuminated with near UV light.

What kind of cell types is RPMI medium used for?

RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

What does RPMI test for?

RPMI is a media for holding tissue for possible flow cytometry studies. When do you use it? Typical usage is for lymph node tissue in a case where the diagnosis is to rule out lymphoma. How much tissue is needed for the test?

What does phenol red test for?

gram negative enteric bacteria
What is this? Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose).

What tests use phenol red?

WHAT IS PHENOL RED? Phenol red is a water-soluble dye used as a pH indicator, changing from yellow to red over pH 6.6 to 8.0, and then turning a bright pink color above pH 8.1. As such, phenol red can be used as a pH indicator dye in various medical and cell biology tests.