To isolate actinomycetes, three isolation media such as starch casein (SC), Luria Bertani (M1) and starch nitrate (SN) agar were used. Prior to isolation, the soil samples were heat for 1h at 121°C. In serial dilution method, serial dilution upto 10-6 was carried out.

How are actinomycetes isolated?

To isolate actinomycetes, three isolation media such as starch casein (SC), Luria Bertani (M1) and starch nitrate (SN) agar were used. Prior to isolation, the soil samples were heat for 1h at 121°C. In serial dilution method, serial dilution upto 10-6 was carried out.

Which media is used for isolation of actinomycetes?

Media: the Casein Starch Agar (CSA) medium used for actinomycetes isolation contained (soluble starch 10g, casein 0.3 g, KNO 2 g, NaCl 2 g, K2HPO4 2 g, MgSO4-7H2O 0.05 g, CaCO3 0.02 g, FeSO4-7H2O 0.01 g, agar 18g and distilled water to 1 l (pH 7.2) was used for isolation of actinomycetes [11].

How do you isolate pigmented bacteria?

Pigment produced by the bacteria can be isolated using solvent extraction. These pigments can be further purified and characterized for physical and chemical characteristics using various instrumental-based analytical techniques such as TLC, UV–vis Spectroscopy, FTIR, ESI–MS, NMR HPLC and Gel Permeation Chromatography.

How do you isolate Streptomyces from soil?

Isolation of Streptomyces These soil samples were prepared for isolation of bacterial strains by the standard serial dilution method [20]. One gram of each sample was suspended in 9 ml of distilled water and vortexed. Then, serial dilutions of each sample were carried out up to 10−3 dilution.

How do you isolate a microorganism?

There are two main ways to isolate organisms.

1. Streaking for isolation on an agar plate.
2. The pour plate method.

How do you isolate antibiotic producing bacteria?

Because we want to isolate both bacterial and fungal antibiotic producers, two different kinds of media will be used. Glycerol yeast extract media is for isolation of Streptomyces, while Saboraud dextrose agar medium will be used for the isolation of the fungi.

Which medium is commonly used for cultivation of actinomycetes?

Starch casein agar
Starch casein agar (SCA) medium is commonly employed for growing actinomycetes.

What is bacterial pigment?

Similar to fungi, bacteria also produce a wide range of pigments such as carotenoids, melanin, violacein, prodigiosin, pyocyanin, actinorhodin, and zeaxanthin (Ahmad et al., 2012; Venil et al., 2014).

What does bacterial pigment mean?

[bak′tir·ē·əl ‚pig·mən′tā·shən] (microbiology) The organic compounds produced by certain bacteria which give color to both liquid cultures and colonies.

How do you isolate bacteria in soil?

Weigh out 1 g of the soil sample and add it to the bottle of distilled water. Tightly cap the bottle and shake it to thoroughly mix the solution. Label the sterile test tubes “10^-3,” “10^-4,” “10^-5,” and “10^-6.” Add 9 ml of distilled water to each of the tubes, using one of the pipettes.

How are microorganisms isolated in pour plate?

Another method of separating bacteria is the pour plate method. With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. The melted agar is then poured into an empty plate and allowed to solidify.

What is isolation method in microbiology?

Microbial isolation technique is an attempt to grow microorganisms outside of their natural environment. Separation of microorganisms outside the environment aims to obtain bacterial cultures that are no longer mixed with other bacteria called pure cultures.