Calculating PFU Divide the number of plaques by the dilution factor, (ex. 10-6 for the most diluted sample) toobtain the number of Plaque Forming Units (PFU) in 100 μL of phage mixture. Note: If performing the assay in triplicate, use the average number of plaques from the three plates.

How do you calculate plaque assay from PFU?

Calculating PFU Divide the number of plaques by the dilution factor, (ex. 10-6 for the most diluted sample) toobtain the number of Plaque Forming Units (PFU) in 100 μL of phage mixture. Note: If performing the assay in triplicate, use the average number of plaques from the three plates.

What does the plaque assay determine?

The plaque assay is a well established method for measuring virus concentration as it relates to infectious dose. The assay relies on determining the number of plaque forming units (pfu) created in a monolayer of virus-infected cells.

What technique does the plaque assay use?

Virus quantification by plaque assay. This assay is based on a microbiological method conducted in a plate. Specifically, a confluent monolayer of host cells is infected with the virus at varying dilutions and covered with a semisolid medium, such as agar, to prevent the virus infection from spreading indiscriminately.

What is a plaque reduction assay?

The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension. This is incubated to allow the antibody to react with the virus.

What is a plaque assay made of?

Plaque assay Specifically, a confluent monolayer of host cells is infected with the virus at varying dilutions and covered with a semi-solid medium, such as agar or carboxymethyl cellulose, to prevent the virus infection from spreading indiscriminately.

How are virus titers measured?

What is an infectious viral titer assay? There are multiple methods to measure the amount of virus in the sample, such as Real-Time (RT) PCR, Western Blot, ELISA, and flow cytometry. These methods utilize the amount of viral DNA, RNA, or proteins to quantify the virus.

How do you calculate the number of plaques?

These plaques are patches of dead bacteria, and each plaque represents one virus. Find a plate that has between 30 and 300 plaques and count the exact number of plaques on that plate. Take the number of plaques in on your plate and multiply by 10. If you counted 157 plaques, you would get 1570.