What is peritoneal exudate cells?

Peritoneal exudate fluid is very small in quantity and contains some neutrophilic leukocytes, macrophages and lymphocytes. The peritoneal exudate is therefore usually collected to obtain the leukocytes, especially free macrophages.

What are thioglycollate elicited macrophages?

Thioglycollate-elicited peritoneal cells are a common source of macrophages for various in vitro assays, including stimulation with TLR ligands, cell signaling assays, phagocytosis, toxicology studies, and cytokine/chemokine production.

What are peritoneal cells?

Abstract. The peritoneal cavity is a membrane-bound and fluid-filled abdominal cavity of mammals, which contains the liver, spleen, most of the gastro-intestinal tract and other viscera. It harbors a number of immune cells including macrophages, B cells and T cells.

What was the purpose of using cells from the peritoneal cavity?

The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells.

What are peritoneal macrophages?

Peritoneal macrophages are the macrophages that reside in the peritoneal cavity, a fluid-filled space located between the wall of the abdomen and the organs found in the abdomen. In the absence of peritoneal infection or inflammation, peritoneal macrophages are thought to have anti-inflammatory functions.

How do you get peritoneal macrophages?

To increase macrophage yield, sterile eliciting agents, such as Brewer’s thioglycollate broth or proteose peptone, can be injected into the peritoneal cavity prior to cell harvest. These agents increase monocyte migration into the peritoneum, and therefore increase macrophage yield.

What is peritoneal macrophage?

How do you isolate a macrophage?

Allow wash medium to collect in a sterile 50-ml conical centrifuge tube on ice. Centrifuge cells 10 min at 500 × g, room temperature. Discard supernatant. Resuspend cell pellet in macrophage complete medium by tapping tube and pipetting up and down.